Seminar by Farshid Jalalvand – University of Copenhagen

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Seminar by Farshid Jalalvand

The role of vesiculation in altering the outer membrane proteome of Gram-negative bacteria

Farshid Jalalvand, PhD
Danish National Research and Novo Nordisk Foundations 
Centre for Bacterial Stress Response and Persistence 
University of Copenhagen
Production of outer membrane vesicles (OMV) that are secreted from the cellular surface is a ubiquitous phenomenon observed in all Gram-negative bacteria. These nanoparticles have been implicated in host-pathogen interactions, antibiotic resistance and dissemination of genetic elements. We have in our investigations focused our efforts on understanding the role of OMVs in cell envelope remodelling. Our combined proteomics and molecular biological data show that proteins that are imperative for outer membrane stability and uptake of essential co-factors are excluded from the OMV cargo, whereas proteins involved in host immune modulation and cell wall-synthesis regulation are secreted via vesiculation. Our findings indicate that bacteria, in the absence of sophisticated regulatory machineries at their surface, use cargo-sorted vesiculation as a mean to regulate the physiologically vital content of the outer membrane.